There seem to be several Isaac Hughes and we will need to proceed with caution. Here are details of Isaac Hughes from Loudoun, VA.
There was an Isaac Hughes in Goochland, VA whose mother was Sarah. This is a portion of his will. This Hughes family intermarried with George Walton family, signer of Declaration of Independence.
Will of Isaac Hughes, dated Jan 22, 1758, proved April 24, 1758. Wife
Martha; patty Mosby and said Patty's brother George Walton. Cumberland
Marriage Bonds; George Walton and Martha Hughes, Spinster , Feb 22, 1749;
John Coles and Jane Hughes, widow, Dec 4, 1758; Thomas Poindexter and Ann
Hughes, Joseph Hughes, security, Jan 25, 1755; Daniel Hughes and ma,
daughter of John Murrey, Dec 18, 1788. There is in Goochland the will of
Sarah Hughes, dated Jan 1723. She bequeaths her estate to her granddaughter
Elizabeth Cannon. There are numerous grants of land to persons named
Hughes